Composite cell sheet for periodontal regeneration: crosstalk between different types of MSCs in cell sheet facilitates complex periodontal-like tissue regeneration

• DOI: 10.1186/s13287-016-0417-x
• Publication Date: 2016-11-14T13:42:58Z
• Authors: Hao Zhang; Shiyu Liu; Bin Zhu; Qiu Xu; Yin Ding; Yan Jin
• Keywords: Periodontium; Periodontal fiber; Crosstalk

Abstract Background Tissue-engineering strategies based on mesenchymal stem cells (MSCs) and cell sheets have been widely used for periodontal tissue regeneration. However, given the complexity in structure, regeneration methods using a single species of MSC could not fulfill requirement Methods We researched interaction between ligament (PDLSCs) jaw bone marrow-derived (JBMMSCs), constructed composite sheet comprising both above MSCs to regenerate complex periodontium-like structures nude mice. Results Our results show that by co-culturing PDLSCs JBMMSCs, expressions extracellular matrix (ECM)-related genes proteins were significantly improved MSCs. Further investigations showed that, compared or (CSCS), which comprises these two MSCs, expressed higher levels bone- ECM-related proteins, generated structure more similar native physiologically vivo. Conclusions In conclusion, our demonstrate crosstalk JBMMSCs facilitate structures, providing promising new strategy physiological functional tissue.