Non-invasive imaging reveals conditions that impact distribution and persistence of cells after in vivo administration

• DOI: 10.1186/s13287-018-1076-x
• Publication Date: 2018-11-28T09:01:47Z
• Authors: Lauren Scarfe; Arthur Taylor; Jack Sharkey; Rachel Harwood; Michael Barrow; Joan Comenge; Lydia Beeken; Cai Astley; Ilaria Santeramo; Claire Hutchinson; Lorenzo Ressel; Jon Smythe; Eric Austin; Raphael Levy; Matthew J. Rossei...; Dave J. Adams; Harish Poptani; Brian K. Park; Patricia Murray; Bettina Wilm
• Keywords: Persistence (discontinuity)

Cell-based regenerative medicine therapies are now frequently tested in clinical trials. In many conditions, cell administered systemically, but there is little understanding of their fate, and adverse events often under-reported. Currently, it only possible to assess safety fate preclinical studies, specifically by monitoring animals longitudinally using multi-modal imaging approaches. Here, a suite vivo modalities explore the range human murine cells, we investigate how route administration, type host immune status affect cells.We applied unique platform combining bioluminescence, optoacoustic magnetic resonance different types following biodistribution persistence mice administration into venous or arterial system.Longitudinal analyses (i) suggested that intra-arterial may be more hazardous than intravenous for certain types, (ii) revealed potential mouse mesenchymal stem/stromal (MSC) line form tumours depended on strain (iii) indicated clinically umbilical cord (hUC)-derived MSCs can transiently unexpectedly proliferate when intravenously mice.In order perform an adequate assessment cell-based therapies, thorough post required. The non-invasive used here expose not general organ distribution these also detailed view presence within organs and, importantly, tumourigenic potential. Our observation hUC-MSCs bone marrow (hBM)-derived persisted period some suggests with cells should proceed caution.