Extracellular vesicles derived from human Wharton’s jelly mesenchymal stem cells protect hippocampal neurons from oxidative stress and synapse damage induced by amyloid-β oligomers

• DOI: 10.1186/s13287-019-1432-5
• Publication Date: 2019-11-20T10:03:13Z
• Authors: Victor Bodart-Santos; Luiza R. P. de Ca...; Mariana A. de Godoy; André F. Batista; Leonardo M. Saraiva; Luize G. Lima; Carla Andreia Abreu; Fernanda G. De Fe...; Antonio Galina; Rosalia Mendez-Otero; Sergio T. Ferreira
• Keywords: 0301 basic medicine; Medicine (General); QD415-436; Exosomes; Biochemistry; Hippocampus; Extracellular Vesicles; 03 medical and health sciences; R5-920; Animals; Humans; Wharton Jelly; Neurons; 0303 health sciences; Amyloid beta-Peptides; Research; Mesenchymal Stem Cells; Catalase; Neuroprotection; Rats; 3. Good health; Oxidative Stress; Synapses; Protein Multimerization; Reactive Oxygen Species; Biomarkers

Abstract Background Mesenchymal stem cells (MSCs) have been explored as promising tools for treatment of several neurological and neurodegenerative diseases. MSCs release abundant extracellular vesicles (EVs) containing a variety biomolecules, including mRNAs, miRNAs, proteins. We hypothesized that EVs derived from human Wharton’s jelly would act mediators the communication between hMSCs neurons could protect hippocampal damage induced by Alzheimer’s disease-linked amyloid beta oligomers (AβOs). Methods isolated characterized released mesenchymal (hMSC-EVs). The neuroprotective action hMSC-EVs was investigated in primary cultures exposed to AβOs. Results were internalized culture, this enhanced presence AβOs medium. protected oxidative stress synapse Neuroprotection mediated catalase abolished inhibitor, aminotriazole. Conclusions AβOs, related transfer enzymatically active contained EVs. suggest should be further cell-free therapeutic approach prevent neuronal disease.