Enhanced therapeutic effects of MSC-derived extracellular vesicles with an injectable collagen matrix for experimental acute kidney injury treatment

• DOI: 10.1186/s13287-020-01668-w
• Publication Date: 2020-04-22T15:04:14Z
• Authors: Yue Liu; Jian Cui; Hongfen Wang; Kamal Hezam; Xiaotong Zhao; Haoyan Huang; Shang Chen; Zhibo Han; Zhong-Chao Han; Zhikun Guo; Zongjin Li
• Keywords: Extracellular Vesicles; Matrix (chemical analysis)

Mesenchymal stem cell (MSC)-derived extracellular vesicles (EVs) have been shown to therapeutic potential for ischemic diseases and are considered an alternative therapy. However, the low retention poor stability of EVs post-transplantation in vivo remain obstacle prior clinical application EVs.This study was designed investigate whether collagen matrix could increase further improve effects murine acute kidney injury (AKI) model. were isolated from human placental MSCs (hP-MSC-EVs) encapsulated a matrix. Then, we investigated can prolong vivo, enhancing efficiency AKI.Our results indicated that effectively encapsulate EVs, significantly promote sustained release EVs. Collagen has improved AKI model, which proved by Gaussia luciferase (Gluc) imaging. The remarkably facilitated proliferation renal tubular epithelial cells compared with alone. Moreover, augment hP-MSC-EVs as revealed angiogenesis, fibrosis apoptosis, functional analysis. Finally, found play role inhibiting endoplasmic reticulum (ER) stress.Collagen markedly enhanced augmented AKI. This strategy improving efficacy therapy provides new direction cell-free