Culture and characterization of various porcine integumentary-connective tissue-derived mesenchymal stromal cells to facilitate tissue adhesion to percutaneous metal implants

• DOI: 10.1186/s13287-021-02666-2
• Publication Date: 2021-12-18T12:02:37Z
• Authors: Devaveena Dey; Nicholas G. Fischer; Andrea H. Dragon; Elsa Ronzier; Isha Mutreja; David T. Danielson; Cole J. Homer; Jonathan A. Forsberg; Joan E. Bechtold; Conrado Aparicio; Thomas A. Davis
• Keywords: Integumentary system

Abstract Background Transdermal osseointegrated prosthesis have relatively high infection rates leading to implant revision or failure. A principle cause for this complication is the absence of a durable impervious biomechanical seal at interface hard structure (implant) and adjacent soft tissues. This study explores possibility recapitulating an analogous cellular musculoskeletal-connective tissue interface, which present naturally occurring integumentary tissues where exits skin, such as nail bed, hoof, tooth. Methods Porcine mesenchymal stromal cells (pMSCs) were derived from nine different porcine connective tissues: hoof-associated superficial flexor tendon, molar-associated periodontal ligament, Achilles adipose skin dermis hind limb abdominal regions, bone marrow muscle. For all pMSCs, phenotype, multi-lineage differentiation potential their adhesiveness clinical grade titanium was characterized. Transcriptomic analysis 11 common genes encoding cytoskeletal proteins VIM ( Vimentin ), cell–cell cell–matrix adhesion Vinculin, Integrin β1, β2, CD9, CD151 ECM Collagen-1a1, Collagen-4a1, Fibronectin, Laminin-α5, Contactin-3 ) in early passaged performed using qRT-PCR. Results All tissue-derived pMSCs characterized origin by adherence plastic, expression cell surface markers including CD29 , CD44 CD90 CD105, lack hematopoietic CD11b endothelial CD31 markers. differentiated into osteoblasts, adipocytes chondrocytes, albeit varying degrees, under specific culture conditions. Among eleven evaluated, intermediate filament vimentin found highly expressed pMSC isolated tissues, followed extracellular matrix Fibronectin Collagen-1a1 . Expression highest while Col1agen-1a1 molar tendon marrow, respectively. ranked both multilineage assessments metal. Conclusions These findings support further preclinical research these specific-derived MSCs vivo transdermal osseointegration model.