Exosomal lncRNA TUG1 derived from human urine-derived stem cells attenuates renal ischemia/reperfusion injury by interacting with SRSF1 to regulate ASCL4-mediated ferroptosis

• DOI: 10.1186/s13287-022-02986-x
• Publication Date: 2022-07-15T10:04:24Z
• Authors: Zejia Sun; Jiyue Wu; Qing Bi; Wei Wang
• Keywords: N6-Methyladenosine; HEK 293 cells

Human urine-derived stem cells (USCs)-derived exosomes (USC-Exo) could improve kidney ischemia/reperfusion injury (IRI), while the underlying mechanisms of this protective effect remain unclear.Human USCs and USC-Exo were isolated verified by morphology specific biomarkers. The effects on ferroptosis detected in IRI-induced acute (AKI) model C57BL/6 mice. lncRNA taurine-upregulated gene 1 (TUG1) hypoxia/reoxygenation (H/R)-treated human proximal tubular epithelial (HK-2). interaction SRSF1 TUG1, ACSL4 was checked via RNA pull-down/RIP stability assays. LncRNA TUG1 SRSF1/ACSL4-mediated H/R-treated HK-2 AKI mouse models.USC-Exo treatment improved ameliorated models. rich which suppressed exposed to H/R. Mechanistically, regulates mRNA interacting with RNA-binding protein SRSF1. In addition, upregulation or downregulation partially reversed cells. Further, TUG1's repression mice.Collectively, carried regulated ASCL4-mediated then protected AKI. Potentially, can serve as a promising therapeutic method for IRI-AKI.