Exosomes derived from minor salivary gland mesenchymal stem cells: a promising novel exosome exhibiting pro-angiogenic and wound healing effects similar to those of adipose-derived stem cell exosomes

• DOI: 10.1186/s13287-024-04069-5
• Publication Date: 2024-12-03T17:21:36Z
• Authors: Haibo Xiang; Pengbing Ding; Jiaying Qian; Enhang Lu; Yimou Sun; Seyeon Lee; Zhenkun Zhao; Zhixuan Sun; Zhenmin Zhao
• Keywords: Exosome; Stem Cell Therapy

Minor salivary gland mesenchymal stem cells (MSGMSCs) can be easily extracted and have a broad range of sources. Applying exosomes to wounds is highly promising method for promoting wound healing. Exosomes derived from different cell types been proven enhance healing, with adipose-derived (ADSC)-derived being the most extensively researched. Considering that MSGMSCs advantages such as easier extraction compared ADSCs, should also very type in exosome therapy. However, whether MSGMSC-derived (MSGMSC-exos) promote healing how they compare ADSC-derived (ADSC-exos) process remain unclear. The effects MSGMSC-exos ADSC-exos on angiogenesis were investigated vitro using CCK-8, scratch assays, tube formation assays. Subsequently, promotion by was evaluated vivo full-thickness defect model mice. Immunohistochemistry used verify collagen deposition, angiogenesis, proliferation wound. Immunofluorescence staining performed investigate role modulating inflammatory response Furthermore, proteomic sequencing conducted functional similarities differences between proteomes ADSC-exos, key protein contents verified ELISA. exhibited similar migration, proliferation, human umbilical vein endothelial (HUVECs) vitro, comparable dose-dependent effect. In experiments confirmed healing-promoting functions ADSC-exos. promoted neovascularization maturation blood vessels at level Despite showing less deposition than stronger anti-scar anti-inflammatory effects. Proteomic analysis revealed proteins both relatively conserved, ITGB1 identified critical angiogenesis. Further differential specifically enriched reflected their source tissue. Our study confirms exhibit angiogenesis-promoting involved are conserved. Moreover, show This suggests applications treatment.