PRRSV-infected monocyte-derived dendritic cells express high levels of SLA-DR and CD80/86 but do not stimulate PRRSV-naïve regulatory T cells to proliferate

• DOI: 10.1186/s13567-015-0186-z
• Publication Date: 2015-05-19T12:48:13Z
• Authors: Irene M Rodríguez...; Tobias Käser; Jaime Gómez-Laguna; Benjamin Lamp; Leonie Sinn; Till Rümenapf; Librado Carrasco; Armin Saalmüller; Wilhelm Gerner
• Keywords: CD80; CD86

In vitro generated monocyte-derived dendritic cells (moDCs) have frequently been used to study the influence of porcine reproductive and respiratory syndrome virus (PRRSV) infection on antigen presenting cells. However, obtained results often conflicting in regard expression co-stimulatory molecules interaction with T this we performed a detailed phenotypic characterisation PRRSV-infected moDCs non-infected moDCs. For CD163 CD169, which are involved PRRSV-entry into host cells, our show that prior express high levels but only very low for CD169. Following either PRRSV-1 or PRRSV-2 strains after 24 h, PRRSV-nucleoprotein (N-protein)+ N-protein− derived from same microculture were analyzed swine leukocyte antigen-DR (SLA-DR) CD80/86. N-protein+ consistently expressed higher SLA-DR CD80/86 compared We also investigated proliferation frequency Foxp3+ regulatory present within CD4+ co-cultures. Neither CD3-stimulated nor unstimulated showed differences following co-cultivation infected Our suggest more will lead consistent across different laboratories PRRSV as indicated by major between microculture.