A hybrid protein containing MSP1a repeats and Omp7, Omp8 and Omp9 epitopes protect immunized BALB/c mice against anaplasmosis

• DOI: 10.1186/s13567-018-0503-4
• Publication Date: 2018-01-19T13:15:11Z
• Authors: Alex Sander R. Ca...; Luis André M. Mar...; Pritesh Lalwani; Keila Dayane E. S...; Spartaco Astolphi...; Patricia P. Orlandi; Sabrina Epiphanio; Kelvison F. Viana; Mucio Flavio B. R...; Hidelberto M. Silva; Claudio R. F. Mar...; Paulo A. Nogueira
• Keywords: Anaplasmosis

Anaplasma marginale (A. marginale) has a remarkable impact on livestock production, and an effective vaccine is not currently available due to the inexistence of small animal model. Recently, BALB/c mice were successfully infected with A. marginale, resulting in acute persistent anaplasmosis infection. Here, we designed hybrid protein containing repeats polypeptide 1a from major surface protein-1 complex (MSP1a) common epitopes outer membrane proteins (OMPs) OMP7, OMP8 OMP9 expressed Escherichia coli. Our proof-of-concept assessed vaccinal effectiveness against challenge live bacteria. The MSP1a/OMP7/8/9 immunized BALB/C exhibited strong reduction rickettsemia had no signs or hepatic lesions. In contrast, non-immunized body weight loss associated increases monocyte neutrophil counts. Furthermore, displayed atrophies chronic inflammatory infiltrates spleen increased binucleation hydropic degeneration hepatocytes. findings demonstrated that immunization our induced conferred protection anaplasmosis. Therefore, given evidence protective effect anaplasmosis, designs are potential candidates for rational design subunits.