Identification and characterization of microRNA in the lung tissue of pigs with different susceptibilities to PCV2 infection

• DOI: 10.1186/s13567-018-0512-3
• Publication Date: 2018-02-15T08:30:50Z
• Authors: Ping Zhang; Liyuan Wang; Yanping Li; Ping Jiang; Yanchao Wang; Pengfei Wang; Li Kang; Yuding Wang; Yi Sun; Yunliang Jiang
• Keywords: Circovirus; Swine Diseases; 0301 basic medicine; Swine; [SDV]Life Sciences [q-bio]; Veterinary medicine; High-Throughput Nucleotide Sequencing; Real-Time Polymerase Chain Reaction; 3. Good health; [SDV] Life Sciences [q-bio]; MicroRNAs; 03 medical and health sciences; SF600-1100; Animals; Disease Susceptibility; Circoviridae Infections; Lung; Research Article

Porcine circovirus type 2 (PCV2) is the primary cause of post-weaning multisystemic wasting syndrome (PMWS) and other PCV-associated diseases. According to our previous RNA-sequencing analysis, differences in susceptibility PCV2 infection depended on genetic between Laiwu (LW) Yorkshire × Landrace crossbred (YL) pigs, but cellular microRNA (miRNA) that are differentially expressed LW YL pigs before after remain be determined. In this study, high-throughput sequencing was performed determine abundance differential expression miRNA lung tissues from PCV2-infected PCV2-uninfected pigs. total, 295 known 95 novel were identified, 23 25 significantly vs. and/or The levels ssc-miR-122, ssc-miR-192, ssc-miR-451, ssc-miR-486, ssc-miR-504 confirmed by quantitative real-time PCR (qRT-PCR). Analysis potential targets four up-regulated (i.e., ssc-miR-451 ssc-miR-486) identified pathways genes may important for disease resistance. Among miRNA, ssc-miR-122 can repress protein viral DNA replication down-regulate nuclear factor activated T-cells 5 (NFAT5) aminopeptidase puromycin sensitive (NPEPPS) binding their 3′ untranslated region (3′UTR) PK15 cells. Therefore, indirectly suppress targeting related host immune system, such as NFAT5 NPEPPS.