Suppression of lymphocyte apoptosis in spleen by CXCL13 after porcine circovirus type 2 infection and regulatory mechanism of CXCL13 expression in pigs

• DOI: 10.1186/s13567-019-0634-2
• Publication Date: 2019-02-28T12:03:27Z
• Authors: Gen Liu; Yanchao Wang; Shijin Jiang; Minmin Sui; Changying Wang; Li Kang; Yi Sun; Yunliang Jiang
• Keywords: CXCL13

Porcine circovirus-associated disease (PCVAD) is one of the most serious infectious diseases in pigs worldwide. The primary causative agent PCVAD porcine circovirus type 2 (PCV2), which can cause lymphoid depletion and immunosuppression pigs. Our previous study demonstrated that Laiwu (LW) pigs, a Chinese indigenous pig breed, have stronger resistance to PCV2 infection than Yorkshire × Landrace (YL) In this study, we found YL showed more severe lymphocyte apoptosis higher viral load spleen tissue LW To illustrate differential gene expression between healthy infected spleens, transcriptome profiling tissues from PCV2-infected control was compared by RNA sequencing. A total 90 differentially expressed genes (DEGs) identified, including CD207, RSAD2, OAS1, OAS2, MX2, ADRB3, CXCL13, CCR1, ADRA2C, were significantly enriched ontology (GO) terms related defense response virus cell–cell signaling, another nine DEGs, KLF11, HGF, PTGES3, MAP3K11, XDH, CYCS, ACTC1, HSPH1, RYR2, GO regulation cell proliferation or apoptosis. Among these CXCL13 gene, suppress during infection, down-regulated but not By analysis regulatory elements promoter 3′-untranslated region (3′-UTR) single nucleotide polymorphism (SNP) −1014 G > Sus scrofa microRNA-296-5p (ssc-miR-296-5p) participated regulating infection.