Distinct transcriptome signatures of Helicobacter suis and Helicobacter heilmannii strains upon adherence to human gastric epithelial cells

• DOI: 10.1186/s13567-020-00786-w
• Publication Date: 2020-05-07T12:02:57Z
• Authors: Helena Berlamont; Chloë De Witte; Eva Bauwens; Hannah Min Jou; Richard Ducatelle; Ellen De Meester; Yannick Gansemans; Dieter Deforce; Filip Van Nieuwer...; Freddy Haesebrouck; Annemieke Smet
• Keywords: Helicobacter

Abstract The porcine Helicobacter suis and canine-feline H. heilmannii are gastric species with zoonotic potential. However, little is known about the pathogenesis of human infections these species. To gain more insight into interactions both epithelial cells, we investigated bacterial genes that differentially expressed in a strain after adhesion to cell line MKN7. In vitro -MKN7 binding assays were performed obtain RNA for sequencing analysis. bacteria attached cells (i.e. cases) as well unbound controls) isolated, which prokaryotic was purified sequenced. Differentially identified using DESeq2 package SARTools pipeline R. A list 134 (83 up-regulated 51 down-regulated) 143 (60 83 (p adj ≤ 0.01; fold change ≥ 2) adherent strains, respectively. According BLASTp analyses, only 2 commonly 4 down-regulated pathogens. strains belonged multiple functional classes, indicating evokes pleiotropic adaptive responses. Our results suggest distinct pathways involved colonization . Further research needed elucidate clinical significance findings.