Molecular characterization of a Trichinella spiralis serine proteinase

• DOI: 10.1186/s13567-020-00847-0
• Publication Date: 2020-09-28T07:02:54Z
• Authors: Xin Yue; Xiang Yuan Sun; Fang Liu; Chen Xi Hu; Ying Bai; Qi Da Yang; Ruo Dan Liu; Xi Zhang; Jing Cui; Zhong Quan Wang
• Keywords: Trichinella spiralis; Intestinal mucosa

Abstract The aim of this study was to investigate the biological characteristics and functions a Trichinella spiralis serine proteinase (TsSerp) during larval invasion development in host. full-length TsSerp cDNA sequence cloned expressed Escherichia coli BL21. results RT-PCR, IFA western blotting analyses showed that secretory protein highly at T. intestinal infective larva muscle stages primarily located cuticle, stichosome intrauterine embryos parasite. rTsSerp promoted epithelial cells (IECs) enteric mucosa, whereas an anti-rTsSerp antibody impeded invasion; promotion obstruction roles were dose-dependently related antibodies, respectively. Vaccination mice with elicited remarkable humoral immune response (high levels serum IgG, IgG1/IgG2a, IgE IgM), it also triggered both systemic (spleen) local mucosal mesenteric lymph node (MLN) cellular responses, as demonstrated by significant elevation Th1 cytokines (IFN-γ) Th2 (IL-4) after spleen MLN from vaccinated stimulated rTsSerp. Anti-TsSerp antibodies participated killing destruction newborn larvae via ADCC. exhibited 48.7% reduction adult worms 52.5% larvae. These indicated participates host might be considered potential candidate target antigen develop oral polyvalent preventive vaccines against infection.