Molecular characterization of Glaesserella parasuis strains isolated from North America, Europe and Asia by serotyping PCR and LS-PCR

• DOI: 10.1186/s13567-021-00935-9
• Publication Date: 2021-05-12T18:03:00Z
• Authors: Nubia Macedo; Marcelo Gottschalk; Katrin Strutzberg...; Chao Nguyen Van; Lijun Zhang; Geng Zou; Rui Zhou; Thaire Marostica; Maria Jose Clavijo; Alexander Tucker; Virginia Aragon
• Keywords: 0301 basic medicine; Asia; Haemophilus Infections; Swine; Virulence Factors; Veterinary medicine; Sus scrofa; Polymerase Chain Reaction; Microbiology; Gene; Agricultural and Biological Sciences; 619; Haemophilus parasuis; Serotype; 03 medical and health sciences; VtaA marker; Seroepidemiologic Studies; Virology; SF600-1100; Health Sciences; Prevalence; Genetics; Animals; Serotyping; Bacterial Pathogenesis in Animal and Human Health; Viral Diseases in Livestock and Poultry; Biology; Swine Diseases; Immunology and Microbiology; 0303 health sciences; Virulence; Glaesserella parasuis; Public Health, Environmental and Occupational Health; Life Sciences; LS-PCR; Polymerase chain reaction; 3. Good health; [SDV] Life Sciences [q-bio]; Europe; FOS: Biological sciences; North America; Medicine; Animal Science and Zoology; Global Burden of Group A Streptococcal Diseases; Research Article

Glaesserella parasuis strains were characterized by serotyping PCR, vtaA virulence marker Leader Sequence (LS)-PCR, clinical significance, and geographic region. Overall, the serovars 4, 5/12, 7, 1, 13 most commonly detected. Serovars of greatest relevance systemic isolates that had a higher probability being serovar 13, or 7. In comparison, pulmonary likelihood 2, 14. 5/12 have previously been considered disease-associated, but this study agrees with other recent studies showing 7 is indeed associated G. disease. Serovar 4 illustrated how can varying degrees be obtained from pulmonary, systemic, nasal sites. 8, 9, 15, 10 predominantly samples, which indicates limited significance these serovars. Additionally, internal classified as virulent LS-PCR disease-associated isolates, including Isolates cavity, 6, 10, 11, non-virulent LS-PCR. conclusion, distribution remains constant, few representing isolated affected pigs. Moreover, it was confirmed used for prediction field worldwide.