Screening of potential vaccine candidates against pathogenic Brucella spp. using compositive reverse vaccinology

• DOI: 10.1186/s13567-021-00939-5
• Publication Date: 2021-06-02T10:08:03Z
• Authors: Xiaodong Zai; Ying Yin; Fengyu Guo; Qiaoling Yang; Ruihua Li; Yaohui Li; Jun Zhang; Junjie Xu; Wei Chen
• Keywords: Reverse vaccinology; Proteome; Antigenicity; Intracellular parasite

Abstract Brucella spp. are Gram-negative, facultative intracellular bacteria that cause brucellosis in humans and various animals. The threat of has increased, yet currently available live attenuated vaccines still have drawbacks. Therefore, subunit vaccines, produced using protein antigens having the advantage being safe, cost-effective efficacious, urgently needed. In this study, we used core proteome analysis a compositive RV methodology to screen potential broad-spectrum against 213 pathogenic strains with worldwide geographic distribution. Candidate proteins were scored according six biological features: subcellular localization, antigen similarity, antigenicity, mature epitope density, virulence, adhesion probability. analysis, total 32 candidate picked out. Of these, three selected for assessment immunogenicity preliminary protection mouse model: outer membrane Omp19 (used as positive control), type IV secretion system (T4SS) VirB8, I (T1SS) HlyD. These high degree conservation could induce specific humoral cellular immune responses. Omp19, VirB8 HlyD substantially reduce organ bacterial load B. abortus S19 mice provide varying degrees protection. demonstrated effectiveness unique strategy screening . Further evaluation is needed identify levels conferred by vaccine wild-type species challenge.