A multiepitope vaccine encoding four Eimeria epitopes with PLGA nanospheres: a novel vaccine candidate against coccidiosis in laying chickens

• DOI: 10.1186/s13567-022-01045-w
• Publication Date: 2022-04-01T10:13:27Z
• Authors: ZhengQing Yu; SiYing Chen; JianMei Huang; WenXi Ding; YuFeng Chen; JunZhi Su; RuoFeng Yan; LiXin Xu; XiaoKai Song; XiangRui Li
• Keywords: Eimeria maxima; Eimeria acervulina; Vaccine efficacy; PLGA

With a worldwide distribution, Eimeria spp. could result in serious economic losses to the poultry industry. Due drug resistance and residues, there are no ideal drugs vaccines against food animals. In current study, bioinformatics approach was employed design multiepitope antigen, named NSLC protein, encoding antigenic epitopes of E. necatrix NA4, tenella SAG1, acervulina LDH, maxima CDPK. Thereafter, protective immunity protein along with five adjuvants two nanospheres laying chickens evaluated. Based on humoral immunity, cellular oocyst burden, coefficient growth, optimum adjuvant Furthermore, immune route dosage were also investigated according burden growth. Accompanied by promoted secretion antibodies enhanced CD4+ CD8+ T lymphocyte proportions, proteins entrapped PLGA more effective stimulating than other or nanospheres, indicating that for protein. addition, significantly inhibited growth promotion observed animals vaccinated nanospheres. The results suggested intramucosal containing 300 μg most efficient induce four species. Collectively, loaded antigens potential vaccine candidates avian coccidiosis.