Effects of exosomes derived from Trichinella spiralis infective larvae on intestinal epithelial barrier function

• DOI: 10.1186/s13567-022-01108-y
• Publication Date: 2022-10-22T10:03:44Z
• Authors: Ruibiao Wang; Yuheng Zhang; Jingbo Zhen; Jinpeng Zhang; Zixuan Pang; Xuewei Song; Lihao Lin; Feng Sun; Yixin Lu
• Keywords: Trichinella spiralis; Barrier function; Intestinal epithelium

Muscle larvae of Trichinella spiralis parasitize the host intestinal epithelium. The mechanisms exosomes participating in invasion T. muscle are unclear. Hence, purpose this study was to explore effect derived from infective (TsExos) on barrier function porcine small epithelial cells (IPEC-J2). First, TsExos were successfully obtained, and their ingestion by validated. Furthermore, optimal induction condition determined CCK8 kit, we found that exposure 150 μg/mL for 12/24 h decreased viability IPEC-J2 30%. Based outcome, effects cell biological processes tight junctions studied. After coincubation cells, results showed a significant increase content FITC-dextran levels lactate dehydrogenase (LDH) reactive oxygen species (ROS). rate apoptosis increased 12.57%, nuclear pyknosis rupture observed. induced TsExos, expression IL-1 upregulated, but IL-10, TGF-β, TLR-5, MUC-1 MUC-2 downregulated. TsExo also led decrease ZO-1, CLDN-3, OCLN. In conclusion, involved several cellular processes, they disrupting physiological biochemical hyperactivating innate immunity, damaging junctions.