A co-utilization strategy to consume glycerol and monosaccharides by Rhizopus strains for fumaric acid production

• DOI: 10.1186/s13568-018-0601-8
• Publication Date: 2018-04-30T02:08:05Z
• Authors: Sylwia Kowalczyk; Elwira Komoń-Janc...; Agnieszka Glibowska; Adam Kuzdraliński; Tomasz Czernecki; Zdzisław Targoński
• Keywords: Fumaric acid; Monosaccharide; Rhizopus arrhizus; Rhizopus; Rhizopus oryzae

The ability of Rhizopus oryzae to produce fumaric acid in the presence glycerol and/or various monosaccharides as carbon sources was examined for seventeen different strains this fungi. These were tested shake-flask cultures on media containing and seven carbohydrates, including glucose, fructose, galactose, mannose, xylose, arabinose, rhamnose. An interesting applicationally useful phenomenon observed. This work presents a new approach conventional microbiological method producing acid. In 40 g/l sole source, production reached 0.16–6.1 after 192 h. When used single maximum concentration much higher; example, 19.8 achieved when xylose used. co-fermentation (40 g/l) (20 g/l), post-culture broth contained approx. 28.0 with process yield 0.90 g/g 168 by also increased dual like mannose. However, results obtained glucose-glycerol-based medium did not follow trend, showing instead complete utilization glucose significant consumption, but unexpectedly low final amounts yields. Understanding how utilize may provide alternative avenues fermentation.