Application of CRISPR/Cas9 for biomedical discoveries

• DOI: 10.1186/s13578-015-0027-9
• Publication Date: 2015-06-20T18:33:28Z
• Authors: Sean M. Riordan; Daniel P. Heruth; Li Q. Zhang; Shui Qing Ye
• Keywords: Zinc finger nuclease; Genome Engineering; Guide RNA

The Clustered Regions of Interspersed Palindromic Repeats-Cas9 (CRISPR/Cas9), a viral defense system found in bacteria and archaea, has emerged as tour de force genome editing tool. CRISPR/Cas9 is much easier to customize optimize because the site selection for DNA cleavage guided by short sequence RNA rather than an engineered protein systems zinc finger nucleases (ZFN), transcription activator-like effector (TALEN), meganucleases. Although it still suffers from some off-target effects, been broadly successfully applied biomedical discoveries number areas. In this review, we present brief history development CRISPR focus on application technology discoveries. We then concise concluding remarks future directions fast moving field.