Canonical and early lineage-specific stem cell types identified in planarian SirNeoblasts

• DOI: 10.1186/s13619-021-00076-6
• Publication Date: 2021-10-01T10:12:29Z
• Authors: Kaimeng Niu; Hao Xu; Yuanyi Zhou Xiong; Yun Zhao; Chong Gao; Chris W. Seidel; Xue Pan; Yuqing Ying; Kai Lei
• Keywords: Planarian; Cell fate determination; Cell type; Lineage (genetic)

Abstract Background The pluripotent stem cells in planarians, a model for tissue and cellular regeneration, remain further identification. We recently developed method to enrich piwi-1 + Schmidtea mediterranea , by staining with SiR-DNA Cell Tracker Green, named SirNeoblasts that permits their propagation subsequent functional study vivo. Since traditional enrichment planarian neoblasts Hoechst 33342 generates X1 cells, blocking the cell cycle inducing cytotoxicity, this Green represents complementary technological advance investigation of fate regeneration. However, similarities heterogeneity subtypes between unknown. Results In work, we performed single RNA sequencing comparison differential expression patterns publicly available data. found first all lineage-specific progenitor were present comparable proportions SirNeoblasts. addition, contain an early muscle is unreported X1. Analysis new markers putative identified here, sub-clustering analysis, revealed earlier lineages epidermal, muscular, intestinal, pharyngeal progenitors than have been observed Using gcm as marker, also subpopulation resided previously tgs-1 neoblasts. Knockdown impaired neoblast repopulation, suggesting function Conclusions summary, use will enable broad experimental advances regeneration specification, given possibility transplantation recombinant mutagenized are not afforded rapid versatile system.