MicroRNAs, polyamines, and the activities antioxidant enzymes are associated with in vitro rooting in white pine (Pinus strobus L.)

• DOI: 10.1186/s40064-016-2080-1
• Publication Date: 2016-04-05T23:27:30Z
• Authors: Yunjun Fei; Bo Xiao; Man Yang; Qiong Ding; Wei Tang
• Keywords: Polyamine; Polyamine oxidase

Molecular mechanism of in vitro rooting conifer is not fully understood. After establishment a regeneration procedure eastern white pine (Pinus strobus L.) using mature embryos as explants to induce shoot formation on medium containing 3 μM IAA, 6 BA and TDZ root 0.001-0.05 IBA, TDZ, we have investigated the changes polyamine content activities antioxidant enzymes during P. strobus. Our results demonstrated that putrescine (Put), spermidine (Spd), spermine (Spm) did increase first week supplemented with 0.01 indole-3-acetic acid (IAA), whereas levels Put, Spd, Spm increased 1st-3rd culture then decreased IAA. No such change was observed without Measurement enzyme activity polyphenol oxidase, catalase, peroxidase slightly reached highest peak 3rd-5th culture. Quantitative RT-PCR indicated miR160 7th day, miR162, miR397, miR408 from 21th 35th miR857 miR827 49th day. These enhanced biosynthesis, activity, microRNAs are correlated induction