Effect of mucin 4 allele on susceptibility to experimental infection with enterotoxigenic F4 Escherichia coli in pigs fed experimental diets

Enterotoxigenic Escherichia coli Sanger sequencing
DOI: 10.1186/s40104-019-0366-1 Publication Date: 2019-07-16T23:03:51Z
ABSTRACT
This study investigated the validity of DNA-marker based test to determine susceptibility ETEC-F4 diarrhoea by comparing results two DNA sequencing techniques in weaner pigs following experimental infection with F4 enterotoxigenic Escherichia coli (ETEC-F4). The effects diet and genetic were assessed measuring incidence piglet post-weaning (PWD), faecal E. shedding index. A marker-based targeting mucin 4 gene (MUC4) that encodes fimbria receptor identified as either fully susceptible (SS), partially or mildly (SR), resistant (RR) developing diarrhoea. To further analyse this, was undertaken, a significantly higher proportion C nucleotides observed for RR SR at XbaI cleavage site genotypes when compared SS. However, no significant difference found between genotypes. Therefore, obtained from Sanger retrospectively allocated into genotype (MUC4–), case nucleotide, (MUC4+), G single nucleotide polymorphism site. total 72 (age ~ 21 days), weighing 6.1 ± 1.2 kg (mean SEM), fed 3 different diets: (i) positive control (PC) group supplemented g/kg zinc oxide (ZnO), (ii) negative (NC) (no ZnO HAMSA), (iii) containing 50 high-amylose maize starch product (HAMSA) esterified acetate. At days five six after weaning, all orally infected ETEC (serotype O149:F4; toxins LT1, ST1, ST2 EAST). percentage developed (P = 0.05) MUC4+ MUC4– (50% vs. 26.8%, respectively). Furthermore, had less 0.009) than other diets, however similar > diets. These confirm have prevalence exposure, ZnO, irrespective MUC4 status, reduced Additionally, quantifying distribution may be more reliable identifying genotypic traditional methods.
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