Microbial differences between dental plaque and historic dental calculus are related to oral biofilm maturation stage

• DOI: 10.1186/s40168-019-0717-3
• Publication Date: 2019-07-06T07:02:57Z
• Authors: Irina M. Velsko; James A. Fellows ...; Franziska Aron; Richard W. Hagan; Laurent A. F. Frantz; Louise Loe; Juan Bautista Rod...; Eros Chaves; Chris Gosden; Greger Larson; Christina Warinner
• Keywords: Dental plaque; Oral Microbiome

Dental calculus, calcified oral plaque biofilm, contains microbial and host biomolecules that can be used to study historic microbiome communities responses. calculus does not typically accumulate as much today historically, clinical research studies focus primarily on living dental biofilm. However, reflect different conditions of the differences in characteristics between sample types have yet been systematically explored. Here, we compare profiles modern plaque, establish expected these substrates.Metagenomic data was generated from samples, metagenomic downloaded Human Microbiome Project. Microbial composition functional profile were assessed. Metaproteomic obtained a subset samples. Comparisons microbial, protein, metabolomic revealed distinct taxonomic metabolic but collected healthy teeth periodontal disease-affected teeth. Species co-exclusion related biofilm environment. Proteomic profiling tooth samples contain low levels bacterial virulence proteins robust innate immune response. Correlations proteomic suggest co-preservation lipid membranes membrane-associated proteins.Overall, find there are systematic physiology, recognizing is important for accurate interpretation comparing calculus.