Ultra-sensitive isotope probing to quantify activity and substrate assimilation in microbiomes

• DOI: 10.1186/s40168-022-01454-1
• Publication Date: 2023-02-09T02:03:13Z
• Authors: Manuel Kleiner; Angela Kouris; Marlene Violette; Grace D’Angelo; Yihua Liu; Abigail Korenek; Nikola Tolić; Timo Sachsenberg; Janine McCalder; Mary S. Lipton; Marc Strous
• Keywords: Stable-isotope probing; Biogeochemical Cycle; Assimilation (phonology)

Stable isotope probing (SIP) approaches are a critical tool in microbiome research to determine associations between species and substrates, as well the activity of species. The application these ranges from studying microbial communities important for global biogeochemical cycling host-microbiota interactions intestinal tract. Current SIP approaches, such DNA-SIP or nanoSIMS allow analyze incorporation stable isotopes with high coverage taxa community at single cell level, respectively, however they limited terms sensitivity, resolution throughput.