Antioxidant and antimicrobial activities of lignin-derived products from all steam-exploded palm oil mill lignocellulosic biomass waste

• DOI: 10.1186/s40538-023-00529-x
• Publication Date: 2024-01-04T14:02:06Z
• Authors: Sholahuddin Shola...; Dian Yosi Arinawati; Vinod Kumar Nathan; Chikako Asada; Yoshitoshi Nakamura
• Keywords: Polyphenol; Biomass (ecology); Pulp and paper industry; Biomedical Engineering; Organic chemistry; Biotechnological Production of Vanillin; FOS: Medical engineering; Biochemistry; Lignin; 01 natural sciences; Antioxidants; Food science; Engineering; Biochemistry, Genetics and Molecular Biology; Health Sciences; Low-molecular-weight lignin; Palm oil mill biomass waste; Biology; 0105 earth and related environmental sciences; Biomass conversion; Antimicrobials; S; Antioxidants and Free Radicals in Health and Disease; Botany; Life Sciences; Kernel fibers; Agriculture; Depolymerisation; Lignocellulosic biomass; Agronomy; Chemistry; Physical Sciences; Medicine; Antimicrobial; Kernel shell; Steam explosion; Antioxidant; EFB; Catalytic Valorization of Lignin for Renewable Chemicals; Depolymerization; Biotechnology; DPPH

Abstract Background Steam explosion pretreatment has been proven to be an effective treatment for breaking down the recalcitrant character of lignin–carbohydrate complexes (LCC) in lignocellulosic biomass. This study investigated the production of lignin-derived products from steam-exploded palm oil mill lignocellulosic biomass waste (POMLBW), that is, empty fruit bunches (EFB), kernel shells (KS), and kernel fibers (KF), also known as mesocarp fibers. Steam explosions cause lignin depolymerization, which forms various polyphenols. The low average molecular weight of the steam-exploded lignin-derived products and their antioxidant activities could potentially enhance their antimicrobial activities.

Methods POMLBW was steam-exploded with various degrees of severity factors (R0: 4.03, 4.91, 5.12, 5.35, and 5.65). Steam-exploded POMLBW produces lignin-derived products such as low-molecular-weight lignin (LML) and water-soluble lignin (WSL). Antioxidant activity was evaluated using 0.5 mM 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay. Polyphenol content was evaluated using the Folin–Ciocalteu method. The antimicrobial activity was evaluated using an agar diffusion assay with Gram-positive and Gram-negative bacteria, and the thermal characteristics were evaluated using differential scanning calorimetry and thermogravimetric analysis.

Results WSL and LML resulted in high radical scavenging activity (RSA) of approximately 95% and 80%, with 0.25 g/L and 0.5 g/L of EC50, where the polyphenol amount was 242–448 mg/g (catechin eq.) and 20–117 mg/g (catechin eq.) under all LML and WSL conditions, respectively. The steam-exploded POMLBW had an average molecular weight of 1589–2832 Da, and this condition, including high RSA and polyphenol amounts, was responsible for the high antimicrobial activities of LML against both Gram-positive (Salmonella enterica, Pseudomonas aeruginosa, and Escherichia coli) and Gram-positive (Staphylococcus aureus) bacteria. Additionally, the thermal properties investigations revealed that the glass transition temperature was 80–90 °C (Tg), the melting temperature (Tm) was 338–362 °C, and the start temperature was 101–128 °C at the beginning of mass loss.

Conclusions These results show that the lignin-derived product from steam-exploded POMLBW has the potential for antioxidant (LML and WSL) and antimicrobial (LML) applications with good thermal resistance.

Graphical Abstract