Secretory expression of β-1,3-glucomannanase in the oleaginous yeast Rhodosporidium toruloides for improved lipid extraction

• DOI: 10.1186/s40643-023-00639-2
• Publication Date: 2023-03-02T20:02:32Z
• Authors: Shiyu Liang; Yue Zhang; Liting Lyu; Shuang Wang; Zongbao K. Zhao
• Keywords: Technology; Oleaginous yeast; Cell wall engineering; T; Chemical technology; Research; Rhodosporidium toruloides; β-1,3-glucomannanase; Secretory expression; TP1-1185; Lipid extraction; TP248.13-248.65; Biotechnology

Abstract Lipids produced by oleaginous yeasts are considered as sustainable sources for the production of biofuels and oleochemicals. The red yeast Rhodosporidium toruloides can accumulate lipids to over 70% its dry cell mass. To facilitate lipid extraction, a recombinant β-1,3-glucomannanase, MAN5C, has been applied partially breakdown R. wall. In this study, NP11 was engineered secretory expression MAN5C simplify extraction process. Specifically, cassette contained codon-optimized gene integrated into genome Agrobacterium -mediated transformation. strain NP11-MAN5C found with proper secretion active yet no notable compromise in terms growth production. When cultures were extracted ethyl acetate without any pretreatment, 20% total recovered, 4.3-fold higher than that parental NP11. cells heat-treated followed presence culture broth supernatants, up 93% confirming beneficial effects situ. This study provides new strategy engineer more viable down-stream processes. Graphical