Arsenic trioxide induces cell cycle arrest and affects Trk receptor expression in human neuroblastoma SK-N-SH cells

• DOI: 10.1186/s40659-018-0167-6
• Publication Date: 2018-06-13T03:15:20Z
• Authors: Xilin Xiong; Yang Li; Ling Liu; Kai Qi; Chi Zhang; Yueqin Chen; Jianpei Fang
• Keywords: Trk receptor; Arsenic Trioxide

Arsenic trioxide (As2O3), a drug that has been used in China for approximately two thousand years, induces cell death variety of cancer types, including neuroblastoma (NB). The tyrosine kinase receptor (Trk) family comprises three members, namely TrkA, TrkB and TrkC. Various studies have confirmed TrkA TrkC expression is associated with good prognosis NB, while overexpression can lead to tumor growth invasive metastasis. Previous shown As2O3 inhibit the proliferation human NB line also affect N-Myc mRNA expression. It remains unclear whether regulates Trks purposes treating NB. aim present study was investigate effect on Trk lines its potential therapeutic efficacy. SK-N-SH cells were grown increasing doses at different time points. We cultured cells, which treated samples quantified by immunohistochemistry, cycle analyzed flow cytometry. evaluated real-time PCR analysis. Immunohistochemical analyses indicated over-expressed specifically during stages 1, 2 4S disease progression. increased stage 3 4 significantly arrested G2/M phase. In addition, levels upregulated higher concentrations treatment, notably 48-h treatment period. Our findings suggested achieve maximum appropriate regulation 4S, should be relatively longer delivery times;however, 4, an concentration infusion must carefully determined. induced varying degrees may promising adjuvant current treatments due apoptotic effects.