Lotus leaf flavonoids induce apoptosis of human lung cancer A549 cells through the ROS/p38 MAPK pathway

• DOI: 10.1186/s40659-021-00330-w
• Publication Date: 2021-03-02T12:05:28Z
• Authors: Xiang-Bo Jia; Quan Zhang; Lei Xu; Wen-Jian Yao; Li Wei
• Keywords: Hyperoside

Abstract Background Leaves of the natural plant lotus are used in traditional Chinese medicine and tea production. They rich flavonoids. Methods In this study, leaf flavonoids (LLF) were applied to human lung cancer A549 cells small cell H446 vitro verify effect LLF on apoptosis these through ROS/p38 MAPK pathway. Results had no toxic normal at concentrations up 500 µg/mL, but could significantly inhibit proliferation cells. Flow cytometry showed that induce growth We also found increase ROS MDA levels, decrease SOD activity Furthermore, qRT-PCR western blot analyses upregulate expression p38 (p-p38 MAPK), caspase-3, caspase-9, cleaved caspase-9 Bax downregulate Cu/Zn SOD, CAT, Nrf2, NQO1, HO-1, Bcl-2 HPLC mainly contain five active substances: kaempferitrin, hyperoside, astragalin, phloridzin, quercetin. The apoptosis-inducing came from naturally compounds. Conclusions have shown study is a bioactive substance can vitro, merits further research development.