Physiological conditioning by electric field stimulation promotes cardiomyogenic gene expression in human cardiomyocyte progenitor cells

• DOI: 10.1186/scrt482
• Publication Date: 2014-08-04T13:11:07Z
• Authors: Aida Llucià-Valld...; Benjamin Sanchez; Carolina Soler-Bo...; Carolina Gálvez-M...; Santiago Roura; Cristina Prat-Vidal; Isaac Perea-Gil; Javier Rosell-Ferrer; Ramon Bragos; Antoni Bayes-Genis
• Keywords: Transdifferentiation; GATA4

The optimal cell lineage for cardiac-regeneration approaches remains mysterious. Additionally, electrical stimulation promotes cardiomyogenic differentiation of stimulated cells. Therefore, we hypothesized that conditioning cardiomyocyte progenitor cells (CMPCs) might enrich their cardiovascular potential. CMPCs were isolated from human adult atrial appendages, characterized, and electrically 7 14 days. Electrical modulated gene protein expression, increasing all cardiac markers. GATA-binding 4 (GATA4) early transcription factor was significantly overexpressed (P = 0.008), but also its coactivator myocyte enhancer 2A (MEF2A) upregulated 0.073) under stimulation. Moreover, important structural proteins calcium handling-related genes enhanced. cardioregeneration capability is improved by field Consequently, short-term should be a valid biophysical approach to modify toward cardiogenic phenotype, can incorporated into transdifferentiation protocols. Electrostimulated may best-equipped myocardial integration after implantation.