A novel serum-free medium for the expansion of human mesenchymal stem cells

• DOI: 10.1186/scrt8
• Publication Date: 2010-04-02T06:13:44Z
• Authors: Lucas G Chase; Uma Lakshmipathy; Luis A Solchaga; Mahendra S Rao; Mohan C Vemuri
• Keywords: Fetal bovine serum; Multipotent Stem Cell

Human multipotent mesenchymal stem cell (MSC) therapies are being tested clinically for a variety of disorders, including Crohn's disease, multiple sclerosis, graft-versus-host type 1 diabetes, bone fractures, and cartilage defects. However, despite the remarkable clinical advancements in this field, most applications still use traditional culture media containing fetal bovine serum. The ill-defined highly variable nature remains challenge, hampering both basic human MSC research fields. To date, no reliable serum-free medium MSCs has been available.In study, we developed growth on marrow-derived through investigation parameters primary isolation, multipassage expansion, mesoderm differentiation, cellular phenotype, gene-expression analysis.Similar to that achieved with medium, expanded supplemented recombinant platelet-derived factor-BB (PDGF-BB), fibroblast factor (bFGF), transforming (TGF)-beta1 showed extensive propagation retained phenotypic, colony-forming unit potential. monitor global gene expression, transcriptomes under serum-containing conditions were compared, revealing similar expression profiles. In addition, described supported isolation from marrow aspirate continual propagation.Although is not free xenogeneic components, provides substitute applications, setting stage future applications.