Expansion and differentiation of CD14+CD16− and CD14++CD16+ human monocyte subsets from cord blood CD34+ hematopoietic progenitors

• DOI: 10.1189/jlb.0207117
• Publication Date: 2007-06-27T00:54:16Z
• Authors: Malgorzata Stec; Kazimierz Weglarczyk; Jaroslaw Baran; Ewa Zuba; Bozenna Mytar; Juliusz Pryjma; Marek Zembala
• Keywords: Cord blood; CD16; Monocyte; CD163

Abstract To determine whether monocytes can be generated from CD34+ hematopoietic progenitors in large numbers, cord blood cells were first expanded for 3–10 days X-VIVO 10 medium supplemented with FCS, stem cell factor (SCF), thrombopoietin (TPO), and Flt-3 Ligand (Flt-3L), then differentiated IMDM SCF, Flt-3L, IL-3 M-CSF 7–14 days. These two step cultures resulted up to a 600-fold mean increase of total CD14+ cells. Using this approach, subpopulations obtained: CD14+CD16− CD14++CD16+ occurring at 2:1 ratio. 1.25(OH)2 Vitamin D3 added the differentiation altered ratio by decreasing proportion monocytes. In comparison CD14+CD16−, showed different morphology an enhanced expression CD11b, CD33, CD40, CD64, CD86, CD163, HLA-DR, CCR5. Both secreted TNF IL-12p40 but little or no IL-10. released significantly more IL-12p40, better stimulators MLR less S. aureus phagocytosis. are clearly those present may novel monocyte subsets that represent stages distinct biological function.