Activation-induced accumulation of B and T lymphocyte attenuator at the immunological synapse in CD4+ T cells

• DOI: 10.1189/jlb.0309138
• Publication Date: 2009-11-06T02:54:52Z
• Authors: Takayoshi Owada; Norihiko Watanabe; Mie Oki; Yoshihiro Oya; Yasushi Saito; Takashi Saito; Itsuo Iwamoto; Theresa L Murphy; Kenneth M Murphy; Hiroshi Nakajima
• Keywords: Immunological synapse; Attenuator (electronics)

BTLA, a recently cloned coreceptor expressed on lymphocytes, negatively regulates cell activation by recruiting SHP-1/SHP-2. However, the mechanisms that regulate intracellular localization of BTLA and its trafficking to surface in T cells are still unknown. To determine expression cells, we examined subcellular mouse steady state, as well upon using confocal laser-scanning microscopy. We found was localized mainly Golgi apparatus secretory lysosomes resting CD4(+) cells. also translocated accumulated at immunological synapse TCR stimulation. Furthermore, BTLA-HVEM interaction required for association with lipid rafts. These results indicate accumulation tightly regulated HVEM stimulation deliver efficient inhibitory signals regulation activation.