CCL5-enhanced human immature dendritic cell migration through the basement membrane in vitro depends on matrix metalloproteinase-9

• DOI: 10.1189/jlb.0804464
• Publication Date: 2006-01-25T03:17:57Z
• Authors: Valérie Chabot; Pascale Reverdiau; Sophie Iochmann; Angélique Rico; Delphine Sénécal; Caroline Goupille; Pierre-Yves Sizaret; Luc Sensebé
• Keywords: CCL5; Matrigel

Abstract The proinflammatory chemokine CC ligand 5 (CCL5) is a potent chemoattractant of immature dendritic cells (iDCs). It remains to be elucidated whether CCL5 may also enhance iDC migration through the basement membrane by affecting matrix metalloproteinase (MMP)-9 secretion. In this study, iDCs were differentiated in vitro from human monocytes healthy donors. Zymographic analysis cellular membranes nontreated revealed basal secretion pro- and active MMP-9, whereas only pro-MMP-9 was detected conditioned media. Increasing concentrations significantly enhanced MMP-9 iDCs, peaking at 100 ng/ml, which optimally increased reconstituted (Matrigel™) vitro. CCL5-enhanced occurred early (2 h) maintained least for 10 h. A significant increase mRNA synthesis reverse transcriptase-polymerase chain reaction, 6 h treatment, suggests that effect (0–4 on independent synthesis, more delayed (6–10 could mediated an gene expression. Matrigel assay, reduced specific inhibitors such as tissue inhibitor metalloproteinase-1 or anti-MMP-9 antibody, indicates depends MMP-9. These results suggest under inflammatory conditions, rapidly increasing their