Different effects of adiponectin isoforms in human monocytic cells

• DOI: 10.1189/jlb.0905521
• Publication Date: 2006-01-25T03:17:57Z
• Authors: Markus Neumeier; Johanna Weigert; Andreas Schäffler; Gabriele Wehrwein; Ulf Müller-Ladner; Jürgen Schölmerich; Christian Wrede; Christa Buechler
• Keywords: 0301 basic medicine; 610 Medizin; Apoptosis; AMP-Activated Protein Kinases; Protein Serine-Threonine Kinases; Monocytes; Cell Line; Mice; 03 medical and health sciences; Multienzyme Complexes; endotoxin; inflammation; adipokine; diabetes; Animals; Humans; Protein Isoforms; RNA, Messenger; Phosphorylation; Cells, Cultured; Receptors, Scavenger; Interleukin-6; Anti-Inflammatory Agents, Non-Steroidal; Recombinant Proteins; Interleukin-10; 3. Good health; Molecular Weight; Adiponectin

Abstract Adiponectin (APM) is an adipocyte-derived adipokine with immunosuppressive, antidiabetic, and antiatherosclerotic properties. Low molecular weight (LMW)- higher (HMW)-APM circulate in the serum activate different signaling pathways. We were interested to see whether LMW-APM exerts effects on monocytic cells compared HMW isoform. Therefore, of recombinant produced insect APM from eukaryotic containing forms investigated respect apoptosis inflammation. LMW- HMW-APM induce nondifferentiated THP-1 cells, reduce macrophage scavenger receptor (MSR) A mRNA expression, stimulate phosphorylation adenosine monophosphate-activated protein kinase (AMPK). However, induces secretion interleukin (IL)-6 human monocytes but does not suppress lipopolysaccharide (LPS)-induced IL-6 secretion. In contrast, reduces LPS-mediated release furthermore, stimulates IL-10 secretion, most likely by reducing abundance inhibitor nuclear factor (NF)-κB β, leading a diminished translocation NF-κB p65. Our data indicate that isoforms do share common also isoform-specific responses. Although apoptosis, activation AMPK, reduction MSR are mediated all isoforms, only displays anti-inflammatory