Dynamics of dendritic cell-derived vesicles: high-resolution flow cytometric analysis of extracellular vesicle quantity and quality

• DOI: 10.1189/jlb.0911480
• Publication Date: 2012-12-18T03:20:25Z
• Authors: Esther N M Nolte-...; Els J van der Vlist; Mieke de Boer-Bro...; Ger J A Arkesteijn; Willem Stoorvogel; Marca H M Wauben
• Keywords: Extracellular Vesicles; Extracellular vesicles

Nano-sized membrane vesicles are secreted by many cell types. These can serve as carriers of cellular information. DC-derived be targeted to other immune cells and modify their function. Accurate analysis quantitative qualitative changes in EV production DC upon different activation stimuli is needed further reveal the regulatory properties EVs. However, methods for reliable quantification individual EVs heterogeneity populations limited. With our recently developed high-resolution flow cytometry-based method, we perform a high-throughput, multiparameter, use this novel technique, show that despite previous assumptions, stimulation with bacterial LPS increases release DC. Furthermore, demonstrate regarding buoyant density MHC class II content. Finally, cognate interaction between LPS-stimulated CD4(+) T affects both quantity quality present culture supernatant. data indicate valuable, tool study dynamics secretion composition, offering great opportunities unveil function cell-derived