Dietary fiber and the short-chain fatty acid acetate promote resolution of neutrophilic inflammation in a model of gout in mice

• DOI: 10.1189/jlb.3a1015-453rrr
• Publication Date: 2016-08-06T03:08:51Z
• Authors: Angélica T Vieira; Izabela Galvão; Laurence M Macia; érica M Sernaglia; Marco Aurélio R V...; Cristiana C Garcia; Luciana P Tavares; Flávio A Amaral; Lirlândia P Sousa; Flaviano S Martins; Charles R Mackay; Mauro M Teixeira
• Keywords: Dietary Fiber; Inflammation; Male; 0301 basic medicine; Gout; Neutrophils; Macrophages; NF-kappa B; Apoptosis; Acetates; Diet, High-Fat; Uric Acid; 3. Good health; Mice, Inbred C57BL; Disease Models, Animal; 03 medical and health sciences; Phagocytosis; Caspases; Animals; Joints; Inflammation Mediators; Crystallization

Gout is a disease characterized by the deposition of monosodium urate (MSU) crystals in joints. Continuous gout episodes may lead to unresolved inflammatory responses and tissue damage. We investigated effects high-fiber diet acetate, short-chain fatty acid (SCFA) resulting from metabolism fiber gut microbiota, on response an experimental model mice. Injection MSU into knee joint mice induced neutrophil influx hypernociception. The onset was not altered animals given diet, but faster resolution response. Similar results were obtained SCFA acetate. Acetate effective, even when after injection at peak caspase-dependent apoptosis neutrophils that accounted for inflammation. Resolution neutrophilic inflammation associated with decreased NF-κB activity enhanced production anti-inflammatory mediators, including IL-10, TGF-β, annexin A1. treatment or intake efferocytosis, effect also observed vitro treated In conclusion, one its metabolic products, controls favoring Our studies suggest what we eat plays determinant role our capacity fine tune