Human MAIT-cell responses to Escherichia coli: activation, cytokine production, proliferation, and cytotoxicity

• DOI: 10.1189/jlb.4ta0815-391rr
• Publication Date: 2016-04-01T03:17:30Z
• Authors: Joana Dias; Michał J Sobkowiak; Johan K Sandberg; Edwin Leeansyah
• Keywords: Antigens, Bacterial; 0303 health sciences; Histocompatibility Antigens Class I; Receptors, Antigen, T-Cell; Antigen-Presenting Cells; Lymphocyte Activation; Mucosal-Associated Invariant T Cells; 3. Good health; Minor Histocompatibility Antigens; 03 medical and health sciences; Technical Advance; T-Lymphocyte Subsets; Escherichia coli; Cytokines; Humans; Cells, Cultured; Escherichia coli Infections

Mucosa-associated invariant T cells are a large and relatively recently described innate-like antimicrobial T-cell subset in humans. These recognize riboflavin metabolites from range of microbes presented by evolutionarily conserved major histocompatibility complex, class I-related molecules. Given the characteristics mucosa-associated novel type antigens they recognize, new methodology must be developed existing methods refined to allow comprehensive studies their role human immune defense against microbial infection. In this study, we established protocols examine functions as respond antigen produced Escherichia coli improved dose- time-optimized experimental detailed MR1-dependent responses pulsed antigen-presenting cells, assessed expression activation markers cytokines, proliferation, induction apoptosis death I-related-expressing target cells. The optimized establish framework open possibilities study immunobiology, using model antigen. Furthermore, propose that these robust systems can also adapted other types