MiR-21-5p-expressing bone marrow mesenchymal stem cells alleviate myocardial ischemia/reperfusion injury by regulating the circRNA_0031672/miR-21-5p/programmed cell death protein 4 pathway.

• DOI: 10.11909/j.issn.1671-5411.2021.12.004
• Publication Date: 2021-12-28
• Authors: Jing Zhang; Changjun Luo; Xiaoqi Xiong; ­Jun Li­; San-Hua Tang; Lin Sun; Qiang Su

For patients with coronary heart disease, reperfusion treatment strategies are often complicated by ischemia/reperfusion (I/R) injury (IRI), leading to serious organ damage and malfunction. The miR-21/programmed cell death protein 4 (PDCD4) pathway is involved in the IRI of cardiomyocytes; however, aberrant miR-21 expression remains unexplained. Therefore, this study aimed explore whether circRNA_0031672 downregulates miR-21-5p during I/R determine miR-21-5p-expressing bone marrow mesenchymal stem cells (BMSCs) reduce myocardial IRI.CircRNA_0031672, miR-21-5p, PDCD4 expressions were evaluated rat model hypoxia/re-oxygenation (H/R)-treated H9C2 cells. Their interactions subsequently investigated using luciferase reporter RNA pulldown assays. Methyltransferase-like 3, a methyltransferase catalyzing N6-methyladenosine (m6A), was overexpressed m6A modification influences targeting PDCD4. BMSCs stably expressing co-cultured investigate protective effect on upon H/R.I/R downregulated upregulated expressions. CircRNA_0031672 knockdown increased expression, but repressed indicating that competitively bound prevented it from mRNA. regulated had no circRNA_0031672/miR-21-5p/PDCD4 axis viability apoptosis after H/R treatment; co-culture restored abundance further reduced induced H/R.We identified novel signaling mediates cardiomyocytes successfully alleviates BMSCs, offering insights into pathogenesis cardiovascular diseases.