Plasma levels and zymographic activities of matrix metalloproteinases 2 and 9 in type II diabetics with peripheral arterial disease

• DOI: 10.1191/1358863x05vm582oa
• Publication Date: 2005-04-15T00:46:57Z
• Authors: Salvatore Santo S...; Grazia Malaponte; Massimo Libra; Luigi Di Pino; Gabriella Celotta; Valentina Bevelacqua; Marcello Petrina; Giuseppina S Nicotra; Manuela Indelicato; Patrick M Navolanic; Giuseppe Pennisi; Maria Clorinda Ma...
• Keywords: Male; Peripheral Vascular Diseases; 0301 basic medicine; type II diabetes; MMP-2; vascular disease; Enzyme-Linked Immunosorbent Assay; Middle Aged; MMP-2; MMP-9; Type II diabetes; Vascular disease; 03 medical and health sciences; Diabetes Mellitus, Type 2; Matrix Metalloproteinase 9; Humans; Matrix Metalloproteinase 2; Female; MMP-9; Biomarkers; Diabetic Angiopathies; Aged

Deregulation of matrix metalloproteinases (MMPs) is an important factor contributing to the development of vascular lesions. Plasma levels and zymographic activities of MMP-2 and MMP-9 were investigated in type II diabetics with ( n = 51) or without ( n = 42) peripheral artery disease (PAD) and in normal volunteers ( n = 23). Plasma MMP-2 levels were higher in type II diabetics with ( p < 0.01) or without ( p < 0.05) PAD in comparison with normal volunteers. Similarly, type II diabetics with ( p < 0.0001) or without ( p > 0.05) PAD had higher plasma MMP-9 levels than normal volunteers. Plasma zymographic activities of both MMP-2 and MMP-9 were positively correlated with their plasma levels. Plasma MMP-2 zymographic activity was higher in type II diabetics with PAD than type II diabetics without PAD ( p > 0.05). Plasma MMP-9 zymographic activity was higher in type II diabetics with ( p < 0.0001) or without ( p < 0.0001) PAD in comparision with normal volunteers. Together, these results indicate that increased plasma levels and zymographic activities of MMP-2 and MMP-9 may contribute to PAD in type II diabetics. In particular, plasma MMP-9 may be a useful marker for the development of vascular disease in type II diabetics.