Application of supercritical CO2 for extraction of polyisoprenoid alcohols and their esters from plant tissues

• DOI: 10.1194/jlr.d038794
• Publication Date: 2013-05-15T03:18:49Z
• Authors: Adam Jozwiak; Robert Brzozowski; Zygmunt Bujnowski; Tadeusz Chojnacki; Ewa Swiezewska
• Keywords: Supercritical fluid extraction

In this study, a method of supercritical fluid extraction (SFE) with carbon dioxide polyisoprenoids from plant photosynthetic tissues is described. SFE was an effective for short- and medium-chain compounds even higher yield than that observed the "classical extraction" organic solvents. Moreover, SFE-derived extracts contained lower amounts impurities (e.g., chlorophylls) those obtained by same tissue Elevated temperature extended time resulted in rate long-chain polyisoprenoids. Ethanol cofeeding did not increase efficiency polyisoprenoids; instead, it increased content lipid extract. Optimization gives opportunity prefractionation complex polyisoprenoid mixtures accumulated tissues. Extracts application are very stable free solvents can further be used directly experimental diet supplementation or as starting material preparation semisynthetic derivatives, e.g., phosphates.