Metabolic switching of human myotubes is improved by n-3 fatty acids

• DOI: 10.1194/jlr.m003319
• Publication Date: 2010-04-05T17:44:27Z
• Authors: N.P. Hessvik; S.S. Bakke; K. Fredriksson; M.V. Boekschoten; A. Fj⊘rkenstad; G. Koster; M.K. Hesselink; S. Kersten; E.T. Kase; A.C. Rustan; G.H. Thoresen
• Keywords: Male; cholesterol-metabolism; Hydrocarbons, Fluorinated; Muscle Fibers, Skeletal; QD415-436; induced insulin-resistance; Biochemistry; metabolic flexibility; 03 medical and health sciences; substrate oxidation; lipid metabolism; Fatty Acids, Omega-3; proliferator-activated receptor; Humans; Insulin; Liver X Receptors; Sulfonamides; 0303 health sciences; skeletal-muscle cells; x-receptor lxr; Gene Expression Profiling; type-2 diabetic subjects; adaptability; Biological Transport; Middle Aged; Orphan Nuclear Receptors; Glucose; nutritional regulation; suppressibility; Female; cross-talk; substrate-regulated flexibility; Energy Metabolism; Oxidation-Reduction; weight-loss; Oleic Acid; Signal Transduction

The aim of the present study was to examine whether pretreatment with different fatty acids, as well liver X receptor (LXR) agonist T0901317, could modify metabolic switching human myotubes. n-3 FA eicosapentaenoic acid (EPA) increased suppressibility, ability glucose suppress oxidation. Substrate-regulated flexibility, increase oxidation when changing from a high glucose, low condition ("fed") acid, ("fasted") condition, by EPA and other FAs. Adaptability, capacity increasing availability, enhanced after EPA, linoleic (LA), palmitic (PA). T0901317 counteracted effect on suppressibility adaptability, but it did not affect these parameters alone. per se accumulated less, however, LA, oleic treatment number lipid droplets (LD) in LD volume intensity, mitochondrial mass, were independent pretreatment. Microarray analysis showed that regulated more genes than FAs specific pathways involved carbohydrate metabolism induced only EPA. suggests favorable skeletal muscle utilization.