Global changes in phospholipids identified by MALDI MS in rats with focal cerebral ischemia

• DOI: 10.1194/jlr.m022558
• Publication Date: 2012-07-01T00:17:03Z
• Authors: Selina Rahman Shanta; Chang Soon Choi; Jeong Hwa Lee; Chan Young Shin; Young Jun Kim; Kyun-Hwan Kim; Kwang Pyo Kim
• Keywords: Male; 0301 basic medicine; Principal Component Analysis; 0303 health sciences; Sodium; matrix-assisted laser desorption/ionization; Brain; Down-Regulation; brain imaging; QD415-436; Cerebral Infarction; Biochemistry; Brain Ischemia; Molecular Imaging; Rats; Up-Regulation; Molecular Weight; Rats, Sprague-Dawley; 03 medical and health sciences; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Potassium; Animals; Phospholipids; mass spectrometry

Neuronal membrane phospholipids are highly affected by oxidative stress caused ischemic injury. Thus, it is necessary to identify key lipid components that show changes during ischemia develop an effective approach prevent brain damage from The recent development of MALDI imaging MS (MALDI IMS) makes possible change between damaged and normal regions directly tissues. In this study, we conducted IMS on rat brains injury detected various showed unique distributions areas the brain. Among them, confirmed in such as lysophosphatidylcholine, phosphatidylcholine, phosphatidylethanolamine, sphingomyelin followed MS/MS analysis. These lipids were present high concentrations important for maintenance cellular structure well production second messengers signal transduction. Our results emphasize identification phospholipid markers successfully identified several distinctly located tissue.