Effect of a diet-induced n-3 PUFA depletion on cholinergic parameters in the rat hippocampus

• DOI: 10.1194/jlr.m300079-jlr200
• Publication Date: 2003-07-23T21:14:17Z
• Authors: Sabah Aïd; Sylvie Vancassel; Carine Poumès-Bal...; Sylvie Chalon; Philippe Guesnet; Monique Lavialle
• Keywords: 0301 basic medicine; α-linolenic acid deficiency; microdialysis; [SDV]Life Sciences [q-bio]; acide alpha linolenique; Vesicular Acetylcholine Transport Proteins; Vesicular Transport Proteins; QD415-436; microdialyse; Biochemistry; Hippocampus; Synaptic Transmission; comportement animal; 03 medical and health sciences; acide gras polyinsaturé; Animals; Rats, Wistar; Phospholipids; carence; acide gras; frontal cortex; acide docosahexaénoique; Membrane Transport Proteins; docosahexaenoic acid; Receptors, Muscarinic; acetylcholine; Acetylcholine; hamster; Diet; Frontal Lobe; Rats; [SDV] Life Sciences [q-bio]; cricetus cricetus; cortex frontal; Acetylcholinesterase; Fatty Acids, Unsaturated; cerveau; acétylcholine; Female; Carrier Proteins

Because brain membranes contain large amounts of docosahexaenoic acid (DHA, 22:6n-3), and as (n-3) PUFA dietary deficiency can lead to impaired attention, learning, memory performance in rodents, we have examined the influence an PUFA-deprived diet on central cholinergic neurotransmission system. We focused several neurochemical parameters frontal cortex hippocampus rats fed PUFA-deficient diet, compared with a control diet. The resulted changes membrane phospholipid compositions both regions, dramatic loss (62-77%) DHA. However, pathway was only modified not cortex. basal acetylcholine (ACh) release deficient significantly (72%) higher than controls, whereas KCl-induced lower (34%). deprivation also caused 10% reduction muscarinic receptor binding. In contrast, acetylcholinesterase activity vesicular ACh transporter regions were unchanged. Thus, evidenced that affect neurotransmission, probably via composition.