An ELISA for apolipoprotein M reveals a strong correlation to total cholesterol in human plasma

• DOI: 10.1194/jlr.m700113-jlr200
• Publication Date: 2007-05-26T01:19:56Z
• Authors: Olof Axler; Josefin Ahnström; Björn Dahlbäck
• Keywords: High-density lipoprotein; Reference range

Apolipoprotein M (apoM) is a 188 amino acid, 25 kDa protein belonging to the lipocalin superfamily. Although predominantly associated with high density lipoprotein, apoM found in all major lipoprotein classes. To facilitate clinical studies of apoM, we have developed sandwich ELISA for measurement human plasma. This method has been used investigate normal variation and establish reference values healthy individuals through 598 samples from Nordic Reference Interval Project Bio-bank Database (NOBIDA) biobank. For women 18-49 years old, interval was 0.58-1.18 micromol/l, whereas 50+ men, range 0.61-1.30 micromol/l. Correlation 26 common chemical analytes NOBIDA database revealed marked positive correlation plasma total cholesterol (r = 0.52) LDL HDL 0.43 0.36, respectively). There no statistically significant HDL/total ratio or body mass index. In conclusion, shows that concentration strongly correlated individuals.