Conjugated linoleic acid-mediated inflammation and insulin resistance in human adipocytes are attenuated by resveratrol

• DOI: 10.1194/jlr.m800258-jlr200
• Publication Date: 2008-09-06T01:41:11Z
• Authors: Arion Kennedy; Angel Overman; Kathleen LaPoint; Robin Hopkins; Tiffany West; Chia-Chi Chuang; Kristina Martinez; Doris Bell; Michael McIntosh
• Keywords: Adult; 0301 basic medicine; Anti-Inflammatory Agents, Non-Steroidal; Fatty Acids; QD415-436; Middle Aged; Transfection; Biochemistry; PPAR gamma; stress; 03 medical and health sciences; delipidation; Resveratrol; Stilbenes; Adipocytes; Humans; Linoleic Acids, Conjugated; Inflammation Mediators; Insulin Resistance; Cells, Cultured; Triglycerides; anti-inflammatory

Inflammation plays a role in <i>trans</i>-10, <i>cis</i>-12 (10,12)-conjugated linoleic acid (CLA)-mediated delipidation and insulin resistance adipocytes. Given the anti-inflammatory of resveratrol (RSV), we hypothesized that RSV would attenuate inflammation caused by 10,12 CLA human blocked induction inflammatory response preventing activation extracellular signal-related kinase gene expression (i.e., IL-6, IL-8, IL-1β) within 12 h. Similarly, suppressed CLA-mediated prostaglandin pathway involving phospholipase A₂, cyclooxygenase-2, PGF_2α. In addition, attenuated increase intracellular calcium reactive oxygen species associated with cellular stress, stress-related proteins activating transcription factor 3, JNK) suppression fatty uptake triglyceride content were RSV. Finally, decrease peroxisome proliferator-activated receptor γ (PPARγ) protein levels proliferator element (PPRE) reporter prevented increased basal activity PPRE, suggesting increases PPARγ activity. Collectively, these data demonstrate for first time prevents adipocytes attenuating stress increasing