Photonic Monitoring in Real Time of Vascular Endothelial Growth Factor Receptor 2 Gene Expression under Relaxin‐Induced Conditions in a Novel Murine Wound Model

• DOI: 10.1196/annals.1282.061
• Publication Date: 2005-06-15T09:26:55Z
• Authors: PETER L. RYAN; RAMEY C. YOUNGBLOOD; JANE HARVILL; SCOTT T. WILLARD
• Keywords: Relaxin; Bioluminescence imaging

A bstract : Relaxin is known to promote vascular endothelial growth factor (VEGF) expression in reproductive tissue, and successful wound healing depends on good vascularization of sites, a process that relaxin may facilitate. Thus, the objective this study was evaluate efficacy development tissue at sites novel VEGF receptor 2‐ luc (VEGFR2‐ ) transgenic mouse model by monitoring rate VEGFR2‐ ‐mediated gene using bioluminescence real‐time imaging. To end, 12 FVB/N male mice were assigned treatments (six per group): saline alone or (1 g/6 h/14 days) administered intraperitoneally (i.p.). On day 0, set full‐thickness wounds (6‐mm punch) generated under anesthesia dorsal aspect each mouse. Photonic emissions recorded (5‐min collection photons) from 10 min after administration luciferin (150 mg/kg i.p.) 0 days 1, 2, 4, 7, 9, 11, 14 postwounding quantify luciferase activity an IVIS 100 biophotonic imaging system. Animals sacrificed (three group) 7 14, specimens recovered for molecular histologic analyses. Although photonic emission increased ( P <.001) over time with peak values obtained no significant >.05) effect treatment noted sites. Whereas measuring relaxin's angiogenesis indirectly via VEGFR2 not successful, provides exciting new tool alternative models (i.e., VEGF‐ mouse) relaxin‐induced normal healing) tumorigenic tissues real time.