Tumor Origin of Endothelial Cells in Human Neuroblastoma

• DOI: 10.1200/jco.2006.09.0696
• Publication Date: 2007-02-02T22:44:49Z
• Authors: Annalisa Pezzolo; Federica Parodi; Maria Valeria Cor...; Roberta Cinti; Claudio Gambini; Vito Pistoia
• Keywords: CD31

Purpose Malignant cells are genetically unstable and prone to develop chemotherapy resistance, whereas tumor vasculature is usually of host origin stable. Tumor endothelial microvessels attract interest as therapeutic targets, but their genetic instability would curtail such approach. Here, we have investigated the in human neuroblastoma (NB). Materials Methods Paraffin-embedded tissue sections from 10 MYCN-amplified tumors (six stage 4, three 3, one 1) were studied. Endothelial (ECs) detected by immunofluorescent staining for CD31 or CD105, MYCN amplification was using fluorescence situ hybridization (FISH). In xenografts HTLA-230 NB cell line, ECs staining, mouse CD34 murine DNA FISH. Results formed approximately 70% two 4 20% 3 tumor. Similar results obtained after EC labeling with CD105. Staining alpha-smooth muscle actin combination FISH demonstrated that tumor-derived coated pericytes. These vessels functional because they contained RBCs. Approximately stained CD31, not CD34, displayed amplification, thus proving origin. Conclusion NB-associated can originate cells, this finding challenges tenet The possibility NB-derived resistant warrants further investigation.