Differential expression of micro-RNA in tumor and non-tumor tissues of patients with colorectal cancer.

• DOI: 10.1200/jco.2020.38.15_suppl.e13516
• Publication Date: 2020-05-25T15:17:10Z
• Authors: Inna A. Novikova; Natalya N. Timosh...; Oleg Ivanovich Kit; Sergey I. Poluektov; Andrey V. Dashkov; Dmitry Haragezov; Roman E. Tolmakh; Evgeniy N. Kolesn...; Vladimir A. Dontsov; Dmitry S. Petrov; Natalya V. Soldat...; Yuriy A. Gevorkyan; Denis S. Kutilin
• Keywords: 03 medical and health sciences; 0302 clinical medicine; 3. Good health

e13516 Background: The colorectal cancer (CRC) incidence is steadily increasing. Moreover, the problem of its early diagnosis remains unresolved due to low specificity known tumor markers, and creating new therapeutic approaches lack a complete understanding mechanisms regulation gene expression in this oncopathology. study micro-RNAs (short non-coding RNAs that regulate expression) can be solution both problems. aim was analyze micro-RNA differential non-tumor tissues CRC patients. Methods: 5 patients with (colon adenocarcinoma, G2) were selected for multiple parallel sequencing. mirVana miRNA Isolation Kit protocol used isolate small RNA fractions. library prepared using TruSeq Small RNASample Preparation Kit. Sequencing nucleotide sequences cDNA libraries performed MiSeq (Illumina, USA). copy numbers determined by comparing sequence sequenced molecules each sample presented databases. When analyzing micro-RNA, DESeq2 method implemented R medium used. Results: Six differentially expressed detected (p < 0.05): 2 decrease (hsa-miR-143-3p,hsa-miR-26a-5p) 4 increase relative (hsa-miR-25-3p, hsa-miR-92a-3p, hsa-miR-21-5p, hsa-let-7i-5p). highest level tissue observed hsa-miR-143-3p, lowest one hsa-let-7i-5p. largest difference shown hsa-miR-92a-3p (4.5 times, p = 0.02), smallest hsa-miR-143-3p (2.4 0.04). For miRNAs changed their expression, search made target genes miRWalk 3.0 database. 14573 found, which 3346 hypo-expressed 11228 hyper-expressed micro-RNAs. Conclusions: revealed 6 (hsa-miR-143-3p, hsa-miR-26a-5p, hsa-miR-25-3p, hsa-let-7i-5p) relatively colon. data obtained expand context oncopathology may possibly become basis highly specific markers panel.