Association between cancer stem cell marker ALDH1 and clinical and morphological factors of colorectal cancer prognosis.

• DOI: 10.1200/jco.2021.39.15_suppl.3522
• Publication Date: 2021-06-02T14:02:23Z
• Authors: Elena A. Dzhenkova; Inna A. Novikova; Oleg I. Kit; Elena Yu. Zlatnik; Elena P. Ulianova; Anastasia O. Sitk...; Oksana G. Shulgina; Yuriy A. Gevorkyan; Natalya V. Soldat...; Vladimir E. Koles...; Maksim K. Aleksan...; Andrey V. Dashkov; Sergey I. Poluektov; Dmitry O. Kaymakchi; Ellada A. Mirzoyan; Vladislav K. Pavlov; Maksim A. Vereshchak; Oksana V. Katelni...; Dmitriy A. Savchenko; Roman E. Tolmakh
• Keywords: clone (Java method); Stem cell marker

3522 Background: Cancer stem cells (CSCs) capable of self-sustaining and multipotent differentiation are considered among the most important factors limiting treatment effectiveness. ALDH1 is a marker colorectal cancer (CRC) CSCs; it involved in cell proliferation, determines resistance to alkylating chemotherapeutic agents, also induces epithelial-mesenchymal transition (EMT), which increases invasive metastatic potential tumors. The purpose study was assess association between expression CSC tissues CRC different stages clinical morphological disease prognosis. Methods: included 299 patients (aged 42-86 years, mean age 64.2±1.7) with stage II-IV T1-4N0-2M0-1; histologically verified G1-G3 adenocarcinoma all patients. Tissues surgically removed tumors were studied IHC analysis using mouse monoclonal anti-ALDH1 antibodies (clone B-5, Santa Cruz Biotechnology) diluted 1:1800 Reveal Polyvalent HRP-DAB Detection System. percentage positively stained for tumor assessed. Statistical performed STATISTICA 13.0 program (StatSoftInc., USA). Results: Positive ALDH1+ registered 52.5% patients, negative – 47.5%. Statistically significant observed stage, since increased from II IV (p = 0.003). statistically significantly associated depth invasion 0.018) presence distant metastases < 0.001). No relationship regional lymph node metastasis 0.788). grade 0.001), perineural 0.010) lymphocytic infiltration histological structure 0.979), lymphovascular 0.772) expression. Tumor site not 0.349). Conclusions: demonstrated characteristics CRC, determining tumor, may be an independent prognostic factor new therapeutic target regulation progression process.