MiRNAs hsa-miR-30a-5p and hsa-miR-99b-5p regulate chemokine-mediated immune response in colorectal cancer.

• DOI: 10.1200/jco.2022.40.16_suppl.e15500
• Publication Date: 2022-06-06T16:16:17Z
• Authors: Oleg I. Kit; Anton A. Pushkin; Dmitry Yu. Gvaldin; Inna A. Novikova; Natalya N. Timosh...; Natalya V. Soldat...; Yuriy A. Gevorkyan; Dmitriy S. Petrov; Vladimir E. Koles...; Dmitriy A. Savchenko; Roman E. Tolmakh; Andrey V. Dashkov; Oksana V. Katelni...; Sergei A. Ilchenko; Elena A. Dzhenkova; Evgeniy N. Kolesn...; Aleksey Yu. Maksimov; Aleksandr V. Shap...
• Keywords: 03 medical and health sciences; 0302 clinical medicine; 3. Good health

e15500 Background: The immune response plays a key role in the oncogenesis of colorectal cancer. heterogeneity tumor environment determines need for immunogenetic profiling to identify associations with implementation tumor-specific responses purpose our study was describe expression profiles miRNAs and genes involved regulation Methods: included 18 patients (median age 66 years) diagnosed cancer who were treated at National Medical Research Centre Oncology 2018-2019. Total RNA isolated using TRIzol (Thermo Fisher, USA) followed by treatment DNase 1 (ThermoFisher, USA). Sequencing samples performed two approaches: AmpliSeq Illumina Immune Response Panel (Illumina, TruSeq Small Library Prep Kit -Set A according manufacturer's instructions on NextSeq 550 device (llumina, Results: revealed 168 differentially expressed 46 (p < 0.05). 29 miRNA-mRNA pairs identified. It has been established that signaling mechanism is chemokine pathway (hsa04062). Expression CXCL1 CXCL10 encoding chemokines same name increased cells (logFC = 1.51, p 0.006 logFC 2.67, 0.001, respectively). At time, level hsa-miR-30a-5p hsa-miR-99b-5p, which negatively regulate CXCL10, decreased -2.26, 0.001 -1.57, 0.001). Conclusions: Activation simultaneous loss negative regulators hsa-miR-99b-5p determined tumors NGS-sequencing, seems promising development personalized therapy, based features colon tumors.